A rapid micrqpreparation technique for extraction of DNA-binding proteins from limiting numbers of mammalian cells
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چکیده
منابع مشابه
A rapid micropreparation technique for extraction of DNA-binding proteins from limiting numbers of mammalian cells.
We have developed a simple and rapid method for preparing DNA-binding protein extracts from mammalian cells. The protocol is derived from the large scale procedure of Dignam et al. (1) and utilizes hypotonic lysis followed by high salt extraction of nuclei. The technique described by Dignam has several drawbacks, including the need for large numbers of cells and lengthy incubation and dialysis ...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1991
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/19.9.2499